Cu-loaded polyurethane to reduce ureteral stent microbes adherence and regulation of the inflammation response to RAW264.7

Introduction: Ureteral stents blocked with encrustation are a common clinical complication and affect bacteria colonization inflammatory response. In this study, different concentrations of copper (0.25, 0.5, 1, 1.5, 2 g/L) were immobilized on polyurethane (PU) that showed functionalization microbe resistance regulation the inflammation response to RAW264.7. Methods: X-ray photoelectron spectroscopy (XPS), atomic force microscope (AFM) static water contact angles used analyze surface characterization. Proteus mirabilis test adhesion cells by SEM carried out evaluate antibacterial property Cu-bearing samples. Cell cytotoxicity assay apoptosis obtain acceptable PU-Cu. The morphology was observe occurrence pseudopodia after Would healing Transwell invasion migration recovery macrophages. IL-6 IL-10 secretion pro-/anti-inflammatory cytokines. Results: (AFM), angle measurement confirm successful immobilization Cu PU. Plate counting observation adhered demonstrated performance PU-Cu against increased amount loading in dependent manner. Furthermore, CCK-8 suggested an at 0.25, 1 g/L. observed reduced Wound transwell assays manifested macrophages improved ELISA could regulate cytokines toward anti-inflammatory functionalization.